Due to receiving large volumes of samples from various sources we must standardize tubes and labeling. Samples that are to be run with a Discovery Assay should be aliquoted into 0.5 – 0.65mL snap-cap microcentrifuge tubes. Compatible tubes have the following approximate dimensions: ~3.0 cm high (9/8 inch), 0.9 cm diameter (3/8 inch).
You are not obligated to use the tubes in the following list; we have provided these examples to use as a guide to make sure that you are using a compatible tube:
Examples of Compatible Tubes:
Cat#: 87003-290 (0.65mL)
Cat#: 20170-293 (0.65mL)
0.5 ml Eppendorf Safe-Lock Tubes™
Order #: 022363611
Fisher Sci. Cat#: 05-402-18
Screw top micro tubes:
Cat#: 3743-MTX (500µl 2D Barcoded, V Bottom ScrewTop Tubes, bulk – no racks, see 4900 for racks)
*If you are using tubes that do not fit this description (ie. 1.5mL tubes or Cryo tubes), please indicate this on the Discovery Assay Order Form; a $3 surcharge per sample will be applied to cover extra handling and material costs.
*Please DO NOT Parafilm seal your tubes.
*Using labels or stickers is NOT recommended – labels can come off when frozen.
Required Volumes and Recommended Dilutions:
For cytokine array Discovery Assays all samples are run at the dilution as they are received – If you wish to have your samples assayed at a dilution, please make this dilution prior to shipping your samples (Refer to Table 1 below or the assay listings on our website for recommended aliquot dilutions). For our most common assays, the following table includes suggested dilutions that you can make in your aliquots with standard PBS (pH~7.5) prior to sending your samples. Running a pilot study is recommended to determine the optimal dilution – particularly for variable sample types such as cell cultures, and homogenates.
|Discovery Assay||Volume Required for testing samples in SINGLE||Volume Required for testing samples in DUPLICATE||Recommended Dilution Factor|
|Serum, Plasma, Urine, CSF & BALF||Cell Culture Supernatant, Tissue Homogenate & Cell Lysate|
|Human 64-plex Assay||100µl||150µl||Undiluted (Note, PDGF-AA, PDGF-BB, RANTES are high in blood samples and may require a dilution. EGF in urine must be run separate at a dilution)||Undiluted (Pilot run recommended)|
|Human 41-plex or 23-plex Assay||50µl||75µl||Undiluted (Note, PDGF-AA, PDGF-BB, RANTES are high in blood samples and may require a dilution. EGF in urine must be run separate at a dilution)||Undiluted (Pilot run recommended)|
|Human MMP & TIMP panel||50µl||75µl||Undiluted (Eve Tech makes dilution)||Undiluted (Eve Tech makes dilution)|
|Mouse 32-plex Assay||50µl||75µl||2 fold (25ul sample in 25ul PBS pH~7.5)||Undiluted (Pilot run recommended)|
|Rat 27-plex Assay||50µl||75µl||2 fold (25ul sample in 25ul PBS pH~7.5)||Undiluted (Pilot run recommended)|
|Featured Assays||Check website for required volume||Check website for required volume||Check website for required volume||Check website for required volume|
|Custom-Plex Assays (Pilot study recommended if protocol is not specific with sample type)||Inquire||Inquire||Inquire||Inquire|
Using a permanent marker, label the tube lids/caps alpha numerically with the first letter of your last name followed by consecutive numbers. For example, if your last name is “Smith” and you are sending three samples your tubes will be labeled: S1, S2 and S3. Maintain your own record of which number corresponds to which sample. We do not recommend using stickers or labels as these can come off when frozen. Please DO NOT Parafilm seal your tubes.
Place your samples consecutively in a rack box or cardboard / plastic sample box. The box should then be placed in a leak proof bag with absorbent material. Do NOT put your sample tubes directly into the dry ice. See our shipping guide for more information.