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Human MSD® Discovery Assay® ULTRA HIGH Sensitivity Cytokine Array (MSDUHS)

$82.19

Ultrasensitive assay that simultaneously measures 9 biomarkers associated with the inflammation response and immune system regulation:

IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A, TNF-α

This assay is optimized for analysis of Serum, Plasma, and CSF samples. If you have another sample type, please contact us prior to ordering this assay.

Price
$82.19/sample
(single analysis)

Sample Volume Requirements
Single analysis 50 μL
Duplicate analysis 75 μL

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Species Type

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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type (Fee applies)

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout, fee applies)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions.) We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect. We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

Tube Type:

Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

Consider this assay for ultra-sensitive detection of 9 key biomarkers related to inflammation and immune system regulation in human samples. The Human MSD® Discovery Assay® ULTRA HIGH Sensitivity Cytokine Array (MSDUHS) measures IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A, and TNF-α.

This highly sensitive panel is designed for precise analysis of cytokines even at very low concentrations, making it ideal for studying subtle inflammatory responses and immune system dynamics. It is particularly useful in research areas where detecting minor fluctuations in cytokine levels is crucial, such as in chronic inflammatory conditions, autoimmune diseases, and subtle immune alterations.

With its exceptional sensitivity, this assay enables the detection of cytokine levels in the femtogram range, allowing for accurate analysis even when dealing with extremely low concentrations. This makes it a powerful tool for researchers investigating conditions where cytokine expression may be minimal but still clinically significant. Additionally, this assay is optimized for Serum, Plasma, and CSF samples, ensuring compatibility with a wide range of research needs.

For researchers facing challenges with limited sample volumes, this assay provides a solution without sacrificing sensitivity or accuracy. It is particularly advantageous in scenarios where sample quantity is a constraint, offering reliable results from minimal sample input. The assay’s finely tuned detection capabilities ensure that even the most delicate cytokine trends are captured, making it an invaluable resource for in-depth immunological studies.

Whether you’re studying chronic inflammation, autoimmune responses, or other immune-related conditions, the Human MSD® Discovery Assay® ULTRA HIGH Sensitivity Cytokine Array offers unparalleled precision and reliability. For other sample types or specific research needs, please contact us prior to ordering to ensure optimal assay performance.

Optimized for Serum, Plasma, and CSF samples. If you need to analyze other sample types, please contact us prior to ordering.
 
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Published Research Featuring MSD® ULTRA HIGH Sensitivity Cytokine Array:
  1. Hawerkamp HC, Dyer AH, Patil ND, et al. Characterisation of the pro-inflammatory cytokine signature in severe COVID-19. Frontiers in Immunology, 14, 1170012.
  2. Edén, A., Grahn, A., Bremell, D., et al. (2022). Viral antigen and inflammatory biomarkers in cerebrospinal fluid in patients with COVID-19 infection and neurologic symptoms compared with control participants without infection or neurologic symptoms. JAMA Network Open, 5(5), e2213253.
  3. Carter, M., Casey, S., O’Keeffe, G. W., Gibson, L., & Murray, D. M. (2021). Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: A case-control study. Scientific Reports, 11, 22315.
  4. Zegeye, M. M., Andersson, J. S. O., Wennberg, P., Repsilber, D., Sirsjö, A., & Ljungberg, L. U. (2021). IL-6 as a mediator of the association between traditional risk factors and future myocardial infarction: A nested case-control study. Arteriosclerosis, Thrombosis, and Vascular Biology, 41(4), 1570-1579.