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Human High Sensitivity T-Cell Expanded Panel 21-Plex Discovery Assay® Array (HDHSTC21)

$51.99

Biomarkers
Fractalkine | GM-CSF | IFNγ | IL-1β | IL-2 | IL-4 | IL-5 | IL-6 |  IL-7 | IL-8 | IL-10 | IL-12p70 | IL-13 | IL-17A | IL-21 | IL-23 | ITAC | MIP-1α | MIP-1β | MIP-3α | TNFα

Price
$51.99/sample
(single analysis)

Sample Volume Requirements
Single analysis 75 μL
Duplicate analysis 100 μL

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Species Type

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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type (Fee applies)

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout, fee applies)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions.) We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect. We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

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Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Consider our assay for an in-depth analysis of 21 essential T-cell biomarkers. The Human High Sensitivity T-Cell Expanded Panel 21-Plex Discovery Assay® Array (HDHSTC21) includes key cytokines such as IL-6, IL-17A, and TNFα, providing detailed insights into T-cell mediated immune responses and inflammatory pathways.

Low levels of inflammation are associated with various clinical and sub-clinical diseases, including autoimmune disorders, cardiovascular conditions, diabetes, neurological diseases, cancer and chronic infection. Measuring cytokines at picogram concentrations is crucial for understanding the development and progression of these conditions. For instance, IL-17A and TNFα are critical in studying autoimmune diseases like rheumatoid arthritis and psoriasis, while IL-10 and IL-12p70 play roles in immune regulation and inflammatory responses.

Our assay is also ideal for scenarios where sample volume is limited but high sensitivity is required. With finely tuned detection capabilities in the picogram range, it allows for sample dilution without sacrificing sensitivity, ensuring accurate and reliable results even with constrained volumes.

For even more delicate concentration trends, such as those in the femtogram range, or when working with very limited sample volumes and picogram-level expectations, consider our Human MSD® Discovery Assay® ULTRA HIGH Sensitivity Cytokine Array (MSDUHS). This array offers the highest sensitivity available for precise and accurate cytokine measurement.

Click here for information on shipping biological samples for Discovery Assays    

Published Research Featuring Our HDHSTC21 (HDHSTC14):
    1. Henderson, J. D., Quigley, S. N. Z., Chachra, S. S., Conlon, N., & Ford, D. (2024). The use of a systems approach to increase NAD+ in human participants. npj Aging, 10, Article 7.
    2. Wang, M., Wisniewski, C. A., Xiong, C., Chhoy, P., Goel, H. L., Kumar, A., Zhu, L. J., Li, R., St. Louis, P. A., Ferreira, L. M., Pakula, H., Xu, Z., Loda, M., Jiang, Z., Brehm, M. A., & Mercurio, A. M. (2023). Therapeutic blocking of VEGF binding to neuropilin-2 diminishes PD-L1 expression to activate antitumor immunity in prostate cancer. Science Translational Medicine, 15(694). 
    3. Russo, M. A., Georgius, P., Staats Pires, A., Heng, B., Allwright, M., Guennewig, B., Santarelli, D. M., Bailey, D., Fiore, N. T., Tan, V. X., Latini, A., Guillemin, G. J., & Austin, P. J. (2020). Novel immune biomarkers in complex regional pain syndrome. Journal of Neuroimmunology, 347, 577330.