*NEW* Human Cytokine/Chemokine 48-Plex Discovery Assay® Array (HD48)

We will test your sample(s) and provide results for the following biomarkers:

sCD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A

NOTE: Results will not directly correlate with the previous HD42 or HD65 assay, as a result of the antibody systems being optimized in this new panel. If you are in need of the previous HD42 or HD65 assay in order to correlate with past data, please contact us directly.

Discovery Price $61.99/sample (single analysis – includes sample filtration)

Single analysis
Minimum volume required: 50 uL
Preferred volume: 75 uL

Duplicate analysis
Minimum volume required: 75 uL
Preferred volume: 100 uL

Recommended Dilution: Most samples are run undiluted, however RANTES has high concentrations in serum and plasma. If this target is important to your study and your sample type is serum or plasma, it is recommended to run samples in a separate assay with a dilution factor applied or utilize our “Human Supplemental Biomarker Array” (HDHSB10). Note we also have limited wells in the Featured 3-plex, HPDGR-03-09.

If you expect your samples to have low cytokine levels, i.e. normal healthy populations, you may wish to inquire about a High Sensitivity panel.      

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid

recommend Hyaluronic treatment for better assay performance. $3.99/sample

Tissue Homogenate
Tissue/Cell Culture Supernatant
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel) $3.00 per tube

No
Yes

(Category A)

Yes

(Category B)

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SKU: HD48 Category: Tag:

Description

Biomarker Analytes:

sCD40L, EGF, Eotaxin, FGF-2, Flt-3 Ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A

Biomarker Research Areas:

Immune response, inflammation, allergy, irritable bowel disease (IBD), sepsis, and cancer.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Human Cytokine Array / Chemokine Array 48-Plex facilitates research in any area that involves the immune system such as inflammation, allergies, irritable bowel disease (IBD), sepsis, and cancer. This Discovery Assay® measures forty-eight biomarkers simultaneously in a single microwell. The assay is designed for extracellular protein analysis in nearly all types of biological samples.

Background Information:

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

Intended Sample Type:

Human serum, plasma (EDTA recommended), cell cultures, tissue homogenates, synovial fluid, CSF, sputum, BAL, urine and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane.

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Luminex xMAP technology is used for multiplexed quantification of 48 Human cytokines, chemokines, and growth factors.

The multiplexing analysis is performed using the Luminex™ 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta). Forty-eight markers are simultaneously measured in the samples using Eve Technologies’ Human Cytokine 48-Plex Discovery Assay® (MilliporeSigma, Burlington, Massachusetts, USA) according to the manufacturer’s protocol.  The 48-plex consists of  sCD40L, EGF, Eotaxin, FGF-2, FLT-3 Ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p40), IL-12(p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC, MIG/CXCL9, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, and VEGF-A.  Assay sensitivities of these markers range from 0.14 – 50.78 pg/mL for the 48-plex.  Individual analyte sensitivity values are available in the MILLIPLEX protocol or below.

Cytokine Array Assay Kit Source:

Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy

*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration/low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.

% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-Assay Inter-Assay Accuracy
Analyte pg/mL %CV %CV % Recovery
sCD40L 5.56 <15 <20 95
EGF 3.20 <15 <20 101
Eotaxin 3.08 <15 <20 96
FGF-2 22.3 <15 <20 106
FLT-3L 0.84 <15 <20 95
Fractalkine 29.75 <15 <20 98
G-CSF 3.76 <15 <20 97
GM-CSF 1.55 <15 <20 100
GROα 1.05 <15 <20 99
IFNα2 6.56 <15 <20 98
IFNγ 0.86 <15 <20 94
IL-1α 2.27 <15 <20 98
IL-1β 0.52 <15 <20 82
IL-1RA 1.29 <15 <20 97
IL-2 0.28 <15 <20 95
IL-3 0.28 <15 <20 100
IL-4 0.20 <15 <20 95
IL-5 0.17 <15 <20 99
IL-6 0.14 <15 <20 96
IL-7 0.14 <15 <20 97
IL-8 0.52 <15 <20 96
IL-9 3.05 <15 <20 101
IL-10 0.91 <15 <20 98
IL-12 (p40) 3.24 <15 <20 95
IL-12 (p70) 0.88 <15 <20 96
IL-13 2.58 <15 <20 95
IL-15 0.74 <15 <20 96
IL-17A 0.71 <15 <20 93
IL-17E/IL-25 19.77 <15 <20 93
IL-17F 28.63 <15 <20 95
IL-18 0.53 <15 <20 97
IL-22 12.68 <15 <20 96
IL-27 50.78 <15 <20 96
IP-10 2.13 <15 <20 99
MCP-1 3.05 <15 <20 101
MCP-3 8.61 <15 <20 104
M-CSF 31.95 <15 <20 97
MDC 0.42 <15 <20 96
MIG 3.98 <15 <20 95
MIP-1α 3.82 <15 <20 104
MIP-1β 0.37 <15 <20 99
PDGF-AA 10.33 <15 <20 91
PDGF-AB/BB 16.39 <15 <20 93
RANTES 1.58 <15 <20 93
TGFα 0.97 <15 <20 102
TNFα 5.39 <15 <20 101
TNFβ 0.80 <15 <20 99
VEGF-A 0.98 <15 <20 99

*Values generated from undiluted samples. RANTES may need to be analyzed with a dilution factor due to high concentrations in serum and plasma. Eve Technologies’ “Human Supplemental Biomarker Array 10-plex” Discovery Assay Includes these targets and is run at a 1:100 dilution. It is recommended to use this panel or a custom assay to generate accurate results for these biomarkers.

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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